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legendplex data analysis software suire  (Qognit Inc)

 
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    Qognit Inc legendplex data analysis software suire
    Legendplex Data Analysis Software Suire, supplied by Qognit Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/legendplex+software/analysis+based+cloud+data+legendplex+software/pm42270592-345-5-11
    Average 86 stars, based on 1 article reviews
    legendplex data analysis software suire - by Bioz Stars, 2026-10
    86/100 stars

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    other:

    Article Title: Loading of CAR‐T cells with magnetic nanoparticles for controlled targeting suppresses inflammatory cytokine release and switches tumor cell death mechanism
    Article Snippet: CAR expression of SPION-loaded T cells was investigated 4 and 24 h posttransfection through flow cytometry using Goat F(ab’)2 anti-human IgG-RPE (Southern Biotech, USA).

    Software:

    Article Title: Christensenella minuta protects and restores intestinal barrier in a colitis mouse model by regulating inflammation
    Article Snippet: The samples were analyzed using a flow cytometer Accuri C6 (BD Biosciences, USA). .. Samples were analyzed using LEGENDplex software according to the data analysis software suite manual (legendplex.qognit.com; Supplementary Figure ). ..

    Article Title: CD16dimCD64+ granulocytes are of interest in the diagnosis of ventilator associated pneumonia in neurological intensive care unit patients
    Article Snippet: Background: The diagnosis of ventilator-associated pneumonia (VAP) is often challenging for front-line clinicians.. During bacterial infection, the immune response and cytokine production can lead to emergency haematopoiesis with the release of immature granulocytes (IG).. This cell population can be identified by flow cytometry using a combination of cell surface markers.

    Article Title: HbS promotes TLR4-mediated monocyte activation and proinflammatory cytokine production in sickle cell disease.
    Article Snippet: Department of General Pediatrics and Pediatric Infectious Diseases, Sickle Cell Center, Necker-Enfants Malades Hospital, Assistance Publique-Ho ˇ pitaux de Paris (AP-HP), Université Paris Cité, Paris, France; Laboratory of Cellular and Molecular Mechanisms of Hematological Disorders and Therapeutical Implications, Université Paris Cité, Imagine Institute, Inserm U1163, Paris, France; Laboratory of Excellence GR-Ex, Paris, France; Sorbonne University, CNRS, Institut de Biologie Paris-Seine (IBPS), Protein Engineering Platform, Molecular Interaction Service, Paris, France; and Department of Hematology, Necker-Enfants Malades Hospital, AP-HP, Université Paris Cité, Paris, France publications.org/bloodf/140/18/1972/2052087/blood_b KEY PO INT S

    Article Title: Nanomaterials Trigger Functional Anti-Tumoral Responses in Primary Human Immune Cells.
    Article Snippet: Cytokines were quantified by custom LEGENDplex (5-plex, BioLegend # 740 510) with the Attune NxT Flow Cytometer. .. Cytokine assays were analyzed using the LEGENDplex software Qognit (https://legendplex.qognit.com). .. Statistics: Statistical analyses were conducted using GraphPad Prism 9.5.


    Article Title: Christensenella minuta protects and restores intestinal barrier in a colitis mouse model by regulating inflammation.
    Article Snippet: The samples were analyzed using a flow cytometer Accuri C6 (BD Biosciences, USA). .. Samples were analyzed using LEGENDplex software according to the data analysis software suite manual (legendplex.qognit.com; Supplementary Figure 5). ..


    Flow Cytometry:

    Article Title: HbS promotes TLR4-mediated monocyte activation and proinflammatory cytokine production in sickle cell disease.
    Article Snippet: Department of General Pediatrics and Pediatric Infectious Diseases, Sickle Cell Center, Necker-Enfants Malades Hospital, Assistance Publique-Ho ˇ pitaux de Paris (AP-HP), Université Paris Cité, Paris, France; Laboratory of Cellular and Molecular Mechanisms of Hematological Disorders and Therapeutical Implications, Université Paris Cité, Imagine Institute, Inserm U1163, Paris, France; Laboratory of Excellence GR-Ex, Paris, France; Sorbonne University, CNRS, Institut de Biologie Paris-Seine (IBPS), Protein Engineering Platform, Molecular Interaction Service, Paris, France; and Department of Hematology, Necker-Enfants Malades Hospital, AP-HP, Université Paris Cité, Paris, France publications.org/bloodf/140/18/1972/2052087/blood_b KEY PO INT S



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    Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the <t>LegendPlex</t> NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.
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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
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    Image Search Results


    Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the LegendPlex NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.

    Journal: Molecular Therapy Advances

    Article Title: Single-cell and spatial transcriptomic analyses of gene therapy-associated retinal inflammation in non-human primates

    doi: 10.1016/j.omta.2026.201726

    Figure Lengend Snippet: Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the LegendPlex NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.

    Article Snippet: Data were analyzed in the LEGENDplex Data Analysis Software (Qognit, BioLegend).

    Techniques: Expressing, Fluorescence, Single Cell, Gene Expression

    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Journal: Science immunology

    Article Title: Human LFA-1 governs T cell immune surveillance of the skin

    doi: 10.1126/sciimmunol.adz8360

    Figure Lengend Snippet: Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Article Snippet: Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (Qognit).

    Techniques: Flow Cytometry, MANN-WHITNEY, Immunopeptidomics